Sensors, Vol. 22, Pages 708: Photonic Label-Free Biosensors for Fast and Multiplex Detection of Swine Viral Diseases
Sensors, Vol. 22, Pages 708: Photonic Label-Free Biosensors for Fast and Multiplex Detection of Swine Viral Diseases
Sensors doi: 10.3390/s22030708
Authors:
Maribel Gómez-Gómez
Carles Sánchez
Sergio Peransi
David Zurita
Laurent Bellieres
Sara Recuero
Manuel Rodrigo
Santiago Simón
Alessandra Camarca
Alessandro Capo
Maria Staiano
Antonio Varriale
Sabato D’Auria
Georgios Manessis
Athnasios I. Gelasakis
Ioannis Bossis
Gyula Balka
Lilla Dénes
Maciej Frant
Lapo Nannucci
Matteo Bonasso
Alessandro Giusti
Amadeu Griol
In this paper we present the development of photonic integrated circuit (PIC) biosensors for the label-free detection of six emerging and endemic swine viruses, namely: African Swine Fever Virus (ASFV), Classical Swine Fever Virus (CSFV), Porcine Reproductive and Respiratory Syndrome Virus (PPRSV), Porcine Parvovirus (PPV), Porcine Circovirus 2 (PCV2), and Swine Influenza Virus A (SIV). The optical biosensors are based on evanescent wave technology and, in particular, on Resonant Rings (RRs) fabricated in silicon nitride. The novel biosensors were packaged in an integrated sensing cartridge that included a microfluidic channel for buffer/sample delivery and an optical fiber array for the optical operation of the PICs. Antibodies were used as molecular recognition elements (MREs) and were selected based on western blotting and ELISA experiments to ensure the high sensitivity and specificity of the novel sensors. M...
Source: Sensors - Category: Biotechnology Authors: Maribel G ómez-Gómez Carles S ánchez Sergio Peransi David Zurita Laurent Bellieres Sara Recuero Manuel Rodrigo Santiago Sim ón Alessandra Camarca Alessandro Capo Maria Staiano Antonio Varriale Sabato D ’Auria Georgios Manessis Athnasios I. Gelasakis Tags: Article Source Type: research
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