Radioiodination of balsalazide, bioevaluation and characterization as a highly selective radiotracer for imaging of ulcerative colitis in mice.

This work focuses on tracking stomach ulcerative colitis in mice. High labeling yield and radiochemical purity were achieved for the formation of a [125/131I]balsalazide radiotracer at optimum conditions of oxidizing agent content (chloramines-T (Ch-T), 75 μg), substrate amount (100 μg), pH of reaction mixture (6), reaction time (30 min) and temperature (37oC), using radioactive iodine-125 (200-450 MBq). The radiolabeled compound, [125/131I]balsalazide, was stablein serum and saline solutionduring 24 hours. Balsalazide is acting as a peroxisome proliferator-activated receptors (PPAR γ). Biodistribution studies were carried in normal and, ulcerated colon mice. High uptake of 75±1.90 percent injected dose/g organ(ID/g) observed in ulcerated mice confirmed the suitability of [131I]balsalazide as a novel radiotracer for ulcerative colitis imaging in mice.
Source: Journal of Labelled Compounds and Radiopharmaceuticals - Category: Biochemistry Authors: Tags: RESEARCH ARTICLE Source Type: research