Crystal structures of FolM alternative dihydrofolate reductase 1 from Brucella suis and Brucella canis

Members of the bacterial genus Brucella cause brucellosis, a zoonotic disease that affects both livestock and wildlife. Brucella are category B infectious agents that can be aerosolized for biological warfare. As part of the structural genomics studies at the Seattle Structural Genomics Center for Infectious Disease (SSGCID), FolM alternative dihydrofolate reductases 1 from Brucella suis and Brucella canis were produced and their structures are reported. The enzymes share ∼ 95% sequence identity but have less than 33% sequence identity to other homologues with known structure. The structures are prototypical NADPH-dependent short-chain reductases that share their highest tertiary-structural similarity with protozoan pteridine reductases, which are being investigated for rational therapeutic development.

Source: Acta Crystallographica Section F - December 17, 2021 Category: Biochemistry Authors: Porter, I. Neal, T. Walker, Z. Hayes, D. Fowler, K. Billups, N. Rhoades, A. Smith, C. Smith, K. Staker, B.L. Dranow, D.M. Mayclin, S.J. Subramanian, S. Edwards, T.E. Myler, P.J. Asojo, O.A. Tags: oxidoreductases short-chain dehydrogenase/reductase family dihydrofolate reductases NADPH Brucella suis Brucella canis Seattle Structural Genomics Center for Infectious Disease SSGCID research communications Source Type: research