Biocatalytic self-assembled synthetic vesicles and coacervates: From single compartment to artificial cells

Adv Colloid Interface Sci. 2021 Nov 25;299:102566. doi: 10.1016/j.cis.2021.102566. Online ahead of print.ABSTRACTCompartmentalization is an intrinsic feature of living cells that allows spatiotemporal control over the biochemical pathways expressed in them. Over the years, a library of compartmentalized systems has been generated, which includes nano to micrometer sized biomimetic vesicles derived from lipids, amphiphilic block copolymers, peptides, and nanoparticles. Biocatalytic vesicles have been developed using a simple bag containing enzyme design of liposomes to multienzymes immobilized multi-vesicular compartments for artificial cell generation. Additionally, enzymes were also entrapped in membrane-less coacervate droplets to mimic the cytoplasmic macromolecular crowding mechanisms. Here, we have discussed different types of single and multicompartment systems, emphasizing their recent developments as biocatalytic self-assembled structures using recent examples. Importantly, we have summarized the strategies in the development of the self-assembled structure to improvise their adaptivity and flexibility for enzyme immobilization. Finally, we have presented the use of biocatalytic assemblies in mimicking different aspects of living cells, which further carves the path for the engineering of a minimal cell.PMID:34864354 | DOI:10.1016/j.cis.2021.102566
Source: Advances in Colloid and Interface Science - Category: Chemistry Authors: Source Type: research