Production of both l ‐ and d‐ N‐acyl‐homoserine lactones by Burkholderia cepacia and Vibrio fischeri

Production ofd,l-N-acyl-homoserine lactones (AHLs) byBurkholderia cepacia andVibrio fischeri was monitored over time, with significant amounts ofd-AHLs detected. Quorum sensing (QS) ofV. fischeri was observed with maximum bioluminescence correlating with the maximum concentrations of bothl- andd- octanoyl-homoserine lactones (l- andd-OHL). AbstractQuorum sensing (QS) is a complex process in which molecules, such asl-N-acyl-homoserine lactones (l-AHLs), are produced as essential signaling molecules allowing bacteria to detect and respond to cell population density by gene regulation. Few studies have considered the natural production and role of the opposite enantiomers,d-AHLs. In this work, production ofd,l-AHLs byBurkholderia cepacia andVibrio fischeri was monitored over time, with significant amounts ofd-AHLs detected. Bioluminescence ofV.fischeri was observed with maximum bioluminescence correlating with the maximum concentrations of bothl- andd- octanoyl-homoserine lactones (l- andd-OHL).l-Methionine, a precursor tol-AHLs, was examined via supplementation studies conducted by growing three parallel cultures ofB.cepacia in M9  minimal media with addedl-,d-, ord,l-methionine and observing their effect on the production ofd,l-AHL byB. cepacia. The results show that addition of any methionine (l-,d-, ord,l-) does not affect the overall ratio ofl- tod-AHLs, that isd-AHL production was not selectively enhanced byd-methionine addition. However, the overall AHL (l- andd-) concen...
Source: MicrobiologyOpen - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research