Molecules, Vol. 26, Pages 6298: Effect of the Gintonin-Enriched Fraction on Glucagon-Like-Protein-1 Release

Molecules, Vol. 26, Pages 6298: Effect of the Gintonin-Enriched Fraction on Glucagon-Like-Protein-1 Release Molecules doi: 10.3390/molecules26206298 Authors: Rami Lee Sun-Hye Choi Han-Sung Cho Hongik Hwang Hyewhon Rhim Hyoung-Chun Kim Sung-Hee Hwang Seung-Yeol Nah Ginseng-derived gintonin reportedly contains functional lysophosphatidic acids (LPAs) as LPA receptor ligands. The effect of the gintonin-enriched fraction (GEF) on in vitro and in vivo glucagon-like protein-1 (GLP-1) secretion, which is known to stimulate insulin secretion, via LPA receptor(s) remains unclear. Accordingly, we examined the effects of GEF on GLP-1 secretion using human enteroendocrine NCI-H716 cells. The expression of several of LPA receptor subtypes in NCI-H716 cells using qPCR and Western blotting was examined. LPA receptor subtype expression was in the following order: LPA6 > LPA2 > LPA4 > LPA5 > LPA1 (qPCR), and LPA6 > LPA4 > LPA2 > LPA1 > LPA3 > LPA5 (Western blotting). GEF-stimulated GLP-1 secretion occurred in a dose- and time-dependent manner, which was suppressed by cAMP-Rp, a cAMP antagonist, but not by U73122, a phospholipase C inhibitor. Furthermore, silencing the human LPA6 receptor attenuated GEF-mediated GLP-1 secretion. In mice, low-dose GEF (50 mg/kg, peroral) increased serum GLP-1 levels; this effect was not blocked by Ki16425 co-treatment. Our findings indicate t...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research