GSE185340 Next Generation Sequencing Facilitates Quantitative Analysis of Stat3f/f K5cre Skin Keratinocytes Transcriptomes

Contributors : Kouske Miyauchi ; Masato KuboSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusAtopic dermatitis (AD) is the most common inflammatory skin disease. Stat3 mutation is a major cause of hyper IgE syndrome (HIES), which consistently represent AD like eczematous dermatitis. Based on multi-dimensional transcriptome analysis in pre- and post-flares skin, dermatitis phenotype was controlled by sequential two steps of Stat3 deficiency and environmental pathogenic stimuli. The Stat3 deficiency determined the barrier integrity that lowered threshold of inflammation, but this step was not sufficient to form pathogenicity. Transcriptome data indicated that emergence of dermatitis phenotype need to trigger robust activation of NF B pathway and TH2 cells. Continuous colonization of Staphylococcus aureus was an environmental stimulus to lower the activation threshold of TH2 inflammation in the skin.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

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Atopic dermatitis (AD) is a multifactorial disease associated with epidermal barrier disruption and inflammatory type 2 immune reaction. A microbial dysbiosis characterized by a dominance of Staphylococcus aureus (S. aureus) is also well documented. In earlier studies, in vitro models reproducing some features of AD have been developed by challenging epidermis with either interleukin (IL) cocktails or S. aureus extracts, or by silencing the expression of pivotal epidermal barrier genes. But none of them reproduced the whole pathophysiological AD features.
Source: Journal of Investigative Dermatology - Category: Dermatology Authors: Source Type: research
Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by a dysregulated immune response associated with barrier dysfunction and dysbiosis of the skin microbiota characterized by Staphylococcus aureus colonization in lesional skin. The aim of our study was to compare non-lesional and lesional AD skin transcriptional profile depending on staphylococcal colonization. Skin biopsies were analyzed by RT-qPCR in moderate and severe adults AD patients. Staphylococcus epidermidis was detected in all lesional skin, in greater abundance than in non- lesional skin, while S.
Source: Journal of Investigative Dermatology - Category: Dermatology Authors: Source Type: research
The aim of this study is to deeply investigate probiotic strains efficacy in the containment of the opportunistic pathogen Staphylococcus aureus, involved in several diseases, such as non melanoma skin cancer (i.e. actinic keratosis and squamous cell carcinoma) and those inflammatory ones, such as psoriasis and atopic dermatitis. Indeed, during the onset and progression of these skin disorders, the dysbiosis of the microbiota leads to the reduction of protective commensal resident bacteria, favoring pathogenic/opportunistic species proliferation (including S.
Source: Journal of Investigative Dermatology - Category: Dermatology Authors: Source Type: research
Atopic dermatitis (AD) is an inflammatory dermatosis associated with Staphylococcus aureus (S. aureus) dysbiosis. The immunological mechanisms by which S. aureus contributes to AD remain poorly characterized. We developed a mouse model of AD-like skin inflammation induced by repeated applications of different clinical strains of S. aureus, to explore the role of innate and adaptive immunity in AD. The intensity of the S. aureus-induced AD-like skin inflammation depended on the clinical strain and required the activation of the S.
Source: Journal of Investigative Dermatology - Category: Dermatology Authors: Source Type: research
Atopic dermatitis (AD) and psoriasis (PS) are inflammatory skin diseases and one of the features of AD is dominant colonization with Staphylococcus aureus (S. aureus). Inflammasomes are protein complexes that contribute to host defense against pathogens and cleave the cytokines pro- interleukin (IL)-1 β and pro-IL-18 into their secreted active forms. In the current study, we measured the expression of IL-1 family members and inflammasome components in the skin of PS and AD patients and studied their regulation by cytokines, S.
Source: Journal of Investigative Dermatology - Category: Dermatology Authors: Source Type: research
This article reviews the mechanisms of inflammatory damage of keratinocytes induced by Staphylococcus aureus infection in patients with AD, providing a theoretical basis for the study of new targeted drugs. This review also suggests for the management of Staphylococcus aureus infection in patients with AD.PMID:34585438 | DOI:10.1002/JLB.3MR0921-030RRR
Source: Journal of Leukocyte Biology - Category: Hematology Authors: Source Type: research
J Med Microbiol. 2021 Sep;70(9). doi: 10.1099/jmm.0.001421.ABSTRACTThe pH of skin is critical for skin health and resilience and plays a key role in controlling the skin microbiome. It has been well reported that under dysbiotic conditions such as atopic dermatitis (AD), eczema, etc. there are significant aberrations of skin pH, along with a higher level of Staphylococcus aureus compared to the commensal Staphylococcus epidermidis on skin. To understand the effect of pH on the relative growth of S. epidermidis and S. aureus, we carried out simple in vitro growth kinetic studies of the individual microbes under varying pH c...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Source Type: research
Microbial dysbiosis with increased Staphylococcus aureus (S. aureus) colonization on the skin is a hallmark of atopic dermatitis (AD), however most microbiome studies focus on bacteria in the flexures and the mic...
Source: BMC Microbiology - Category: Microbiology Authors: Tags: Research Source Type: research
Atopic dermatitis (AD) is a common inflammatory skin disease characterized by an epidermal barrier impairment, as well as a Th2/Th22-skewed immune response, both favoring skin colonization with Staphylococcus aureus. Colonization is strongly related to severity of the disease, and a reduction of S. aureus has been found to alleviate symptoms. Lactic acid bacteria (LAB) produce antimicrobial compounds such as organic acids and bacteriocins and are widely used as probiotics. The aim of this study was to isolate LAB and screen for antibacterial effect specifically toward S. aureus clonal complex type 1. A total of 680 LAB wer...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Allergy Asthma Immunol Res. 2021 Sep;13(5):762-775. doi: 10.4168/aair.2021.13.5.762.ABSTRACTPURPOSE: We aimed to investigate the effects of dupilumab on 1) the permeability and antimicrobial barrier, 2) the composition of the skin microbiome, and 3) the correlation between changes in skin barrier properties and microbiota in atopic dermatitis (AD) patients.METHODS: Ten patients with severe AD were treated with dupilumab for 12 weeks. Disease severity was assessed using the Eczema Area and Severity Index (EASI). Skin barrier function was evaluated by measuring transepidermal water loss, stratum corneum (SC) hydration, and p...
Source: Allergy, Asthma and Immunology Research - Category: Allergy & Immunology Authors: Source Type: research
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