Cytotoxin antibody-based colourimetric sensor for field-level differential detection of elapid among big four snake venom

by Sherin Kaul, L. Sai Keerthana, Pankaj Kumar, Komal Birader, Yathirajarao Tammineni, Deepali Rawat, Pankaj Suman Development of a rapid, on-site detection tool for snakebite is highly sought after, owing to its clinically and forensically relevant medicolegal significance. Polyvalent antivenom therapy in the management of such envenomation cases is finite due to its poor venom neutralization capabilities as well as diagnostic ramifications manifested as untoward immunological reactions. For precise molecular diagnosis of elapid venoms of the big four snakes, we have developed a lateral flow kit using a monoclonal antibody (AB1; IgG1– κ chain; Kd: 31 nM) generated against recombinant cytotoxin-7 (rCTX-7; 7.7 kDa) protein of the elapid venom. The monoclonal antibody specifically detected the venoms ofNaja naja (p
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research