Practical Considerations for Using RNA Sequencing in Management of B-Lymphoblastic Leukemia

Despite the immense genetic heterogeneity of B-lymphoblastic leukemia [or precursor B-cell acute lymphoblastic leukemia (B-ALL)], RNA sequencing (RNA-Seq) could comprehensively interrogate its genetic drivers, assigning a specific molecular subtype in>90% of patients. However, study groups have only started to use RNA-Seq. For  broader clinical use, technical, quality control, and appropriate performance validation are needed. We describe the development and validation of an RNA-Seq workflow for subtype classification, TPMT/NUDT15/TP53 variant discovery, and immunoglobulin heavy chain (IGH) disease clone identification for Malaysia-Singapore acute lymphoblastic leukemia (ALL) 2020.

Source: Journal of Molecular Diagnostics - August 5, 2021 Category: Pathology Authors: Winnie Hui Ni Chin, Zhenhua Li, Nan Jiang, Evelyn Huizi Lim, Joshua Yew Suang Lim, Yi Lu, Kean Hui Chiew, Shirley Kow Yin Kham, Bernice Ling Zhi Oh, Ah Moy Tan, Hany Ariffin, Jun J. Yang, Allen Eng-Juh Yeoh Tags: Regular article Source Type: research