Characterization of two pigeon paramyxoviruses type 1 isolates in China.

In this study, two PPMV-1 strains, SD12 and BJ13, obtained from diseased pigeons in China, were characterized. Phylogenetic analysis based on complete sequences allowed characterization of both strains as genotype VI, class II. Further phylogenetic analysis of a 374-nucleotide section of the fusion gene showed that SD12 fell into lineage VIbii-d and BJ13 into VIbii-f. The deduced amino acid sequence of the cleavage site of the fusion protein confirmed that both isolates contained the virulent motif (112)K/RRQKR↓F(117) at the cleavage site. Nevertheless, the values of ICPI showed the SD12 isolate to be a velogenic strain and BJ13 isolate to be a mesogenic strain. The SD12 isolate was further investigated via clinical observation, RNA detection, histopathology, and viral serology in experimentally infected 3-week-old chickens. It showed a mild pathological phenotype in chickens, with viral replication restricted to a few tissues. The molecular mechanism for SD12 isolate with a virulent motif has low levels of virulence for chickens and requires further study.
PMID: 25735628 [PubMed - as supplied by publisher]

Source: Avian Pathology - March 4, 2015 Category: Pathology Authors: Awu A, Shao MY, Liu MM, Hu YX, Qin ZM, Tian FL, Zhang GZ Tags: Avian Pathol Source Type: research