Development and validation of RP-HPLC method for quantification of glipizide in biological macromolecules.

Development and validation of RP-HPLC method for quantification of glipizide in biological macromolecules.
Int J Biol Macromol. 2014 Jan 10;
Authors: Pani NR, Acharya S, Patra S
Abstract
Glipizide (GPZ) has been widely used in the treatment of type-2 diabetics as insulin secretogague. Multiunit chitosan based GPZ floating microspheres was prepared by ionotropic gelation method for gastroretentive delivery using sodiumtripolyphosphate as cross-linking agent. Pharmacokinetic study of microspheres was done in rabbit and plasma samples were analyzed by a newly developed and validated high-performance liquid chromatographic method. Method was developed on Hypersil ODS-18 column using a mobile phase of 10mM phosphate buffer (pH, 3.5) and methanol (25:75, v/v). Elute was monitored at 230nm with a flow rate of 1mL/min. Calibration curve was linear over the concentration range of 25.38 - 2046.45 ng/mL. Retention times of GPZ and internal standard (gliclazide) were 7.32 and 9.02min respectively. Maximum plasma drug concentration, area under the plasma drug concentration-time curve and elimination half life for GPZ floating microspheres were 2.88±0.29μgmL(-1), 38.46±2.26μghmL(-1) and 13.55 ± 1.36h respectively. When the fraction of drug dissolved from microspheres in pH 7.4 was plotted against the fraction of drug absorbed, a linear correlation (R(2) = 0.991) was obtained in in-vitro and in-vivo correlation study.
PMID: 24418334 [PubMed - as...

Source: International Journal of Biological Macromolecules - January 10, 2014 Category: Biochemistry Authors: Pani NR, Acharya S, Patra S Tags: Int J Biol Macromol Source Type: research