Polymorphism analysis of TCP gene region to intraspecific analysis of Paeonia lactiflora, and application for authentication of Paeoniae Radix

AbstractWe attempted to conduct an intraspecific analysis of 30 peony cultivars in Japan and to authenticate five herbal medicine samples derived from Paeoniae Radix by polymorphism analysis of theTEOSINTE BRANCHED1, CYCLOIDEA, and PCF (TCP) gene region. We focused on cultivar-dependent differences in leaf margin undulation and analyzed the sequence of the relatedTCP gene region. As a result, we found that the nucleotide sequences of 29 cultivars ofPaeonia lactiflora except ‘America’ exhibit interspecific variations compared with the nucleotide sequences ofPaeonia suffruticosa andPaeonia tenuifolia. Therefore, in the dendrogram constructed on the basis of the sequence similarity in theTCP gene region, the 29 cultivars ofP. lactiflora were separated fromP. suffruticosa,P. tenuifolia, and ‘America’, and clustered into three subgroups. There were 16 variations containing heterogenous DNA sequences withinP. lactiflora species, and two characteristic variations in subgroup I. SomeP. lactiflora cultivars showed the same nucleotide sequence in theTCP gene region, whereas the five herbal medicine samples showed different sequences, although all of them could be authenticated. The results suggest that Paeoniae Radix in the Japanese crude drug market can be authenticated by analysis of theTCP gene region.Graphical abstract
Source: Journal of Natural Medicines - Category: Drugs & Pharmacology Source Type: research