Practical Considerations for Using RNA Sequencing in Management of B-Cell Acute Lymphoblastic Leukemia

Despite the immense genetic heterogeneity of B-cell acute lymphoblastic leukemia (ALL), RNA sequencing (RNA-Seq) could comprehensively interrogate its genetic drivers, assigning a specific molecular subtype in>90% of patients. However, study groups have only started to use RNA-Seq. For  broader clinical use, technical, quality control, and appropriate performance validation are needed. We describe the development and validation of an RNA-Seq workflow for subtype classification, TPMT/NUDT15/TP53 variant discovery, and IGH disease clone identification for Malaysia-Singapore ALL se quencing (ALL-Seq) 2020.

Source: Journal of Molecular Diagnostics - August 5, 2021 Category: Pathology Authors: Winnie H. Ni Chin, Zhenhua Li, Nan Jiang, Evelyn H. Lim, Joshua Y. Suang Lim, Yi Lu, Kean H. Chiew, Shirley K. Yin Kham, Bernice L. Zhi Oh, Ah M. Tan, Hany Ariffin, Jun J. Yang, Allen Eng-Juh Yeoh Tags: Regular article Source Type: research