Barks of Three Wild Pyrus Taxa: Phenolic Constituents, Antioxidant Activity, and In Vitro and In Silico Investigations of α-Amylase and α-Glucosidase Inhibition

Chem Biodivers. 2021 Aug 16. doi: 10.1002/cbdv.202100446. Online ahead of print.ABSTRACTDry MeOH extracts of the twig barks of Pyrus communis subsp. pyraster, P. spinosa and their hybrid P. ×jordanovii nothosubsp. velenovskyi, collected in wild in Serbia, were analyzed. By LC-MS, the contents of arbutin (99.9-131.0 mg/g), chlorogenic acid (2.2-6.3 mg/g), catechin (1.0-5.3 mg/g) and total dimeric and trimeric procyanidins (42.2-61.3 mg/g), including procyanidin B2 (8.9-17.2 mg/g), were determined. Colorimetrically, high contents of total phenolics (436.2-533.4 mg GAE/g) and tannins (339.4-425.7 mg GAE/g), as well as strong total antioxidant activities (FRAP values 4.5-5.9 mmol Fe2+/g), and DPPH (SC50=6.6-7.1 µg/ml) and hydroxyl radical (SC50=447.1-727.7 µg/ml) scavenging abilities were revealed. In vitro, all extracts exhibited notable inhibition of α-amylase (IC50=310.8-617.7 µg/ml) and particularly strong inhibition of α-glucosidase (IC50=2.1-3.7 µg/ml). Molecular docking predicted that among identified compounds procyanidin B2 is the best inhibitor of these carbohydrate-digesting enzymes. Obtained results showed that the barks of investigated Pyrus hybrid and its parent taxa have similar composition and bioactivity.PMID:34402208 | DOI:10.1002/cbdv.202100446
Source: Chemistry and Biodiversity - Category: Biochemistry Authors: Source Type: research