O-081 High variability of molecular isoforms of AMH in follicular fluid and granulosa cells from human small antral follicles

AbstractStudy questionIs the composition of AMH isoforms different in follicular fluids (FF) and granulosa cells (GCs) from human small antral follicles?Summary answerThere is a high viability of AMH isoforms in FFs and GCs. Even between same size follicles from the same women, the isoform composition differs.What is known alreadyAnti M üllerian Hormone (AMH) is a member of the TGF-β superfamily produced by follicular granulosa cells (GCs) in women from late gestation to the end of reproductive live. AMH is suggested to inhibit aromatase (i.e. CYP19) expression and thereby decreasing the conversion of androgens to oestrogens in h umans, especially in small antral follicles before dominance is achieved and thereby act as a gatekeeper of ovarian steroidogenesis. However, the exact function and processing of AMH in human follicles is still not clarified.Study design, size, durationThis retrospective study measured AMH isoforms in human FF and GCs from small antral follicles using ELISA, Western blot, and immunofluorescence analysis. A total of 41 female adolescents and women aged 15 to 38 years (mean age: 29.7 years), who underwent ovarian tissue cryopreservation (OTC) at the University Hospital of Copenhagen, Rigshospitalet were included between year 2006 and 2020 included.Participants/materials, setting, methodsDonated human ovarian medulla tissue were FFs and GCs were obtained in connection with OTC. The following isoforms were evaluated in FFs using ELISA analysis: full-le...
Source: Human Reproduction - Category: Reproduction Medicine Source Type: research