Analysis of SARS-CoV-2 RT-qPCR Ct values vis- à-vis anti-SARS-CoV-2 antibodies from a high incidence region

We previously reported data concerning anti-SARS-CoV-2 antibodies in PCR-confirmed COVID-19 patients from a high-incidence region in northern Spain (Markewitz et al., 2021). In short, we were able to show that neither the presence nor the level of anti-SARS-CoV-2 specific antibodies served as prognostic markers in our cohort but that they correlated with the amount of time since onset of symptoms. Recently, there has been much debate on the diagnostic value of the na ïve (i.e. without reference to a standard curve) threshold cycle (Ct) results of the reverse transcription PCR (RT-qPCR) that is the gold standard in the laboratory diagnosis of acute cases of COVID-19.
Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Source Type: research