Viruses, Vol. 13, Pages 1093: Genetic Characterization of a New HIV-1 Sub-Subtype A in Cabo Verde, Denominated A8

Viruses, Vol. 13, Pages 1093: Genetic Characterization of a New HIV-1 Sub-Subtype A in Cabo Verde, Denominated A8 Viruses doi: 10.3390/v13061093 Authors: Rayana Katylin Mendes Da Silva Isabel Inês Monteiro de Pina Araujo Karine Venegas Maciera Mariza Gonçalves Morgado Monick Lindenmeyer Guimarães Previous molecular characterization of Human immunodeficiency virus (HIV-1) samples from Cabo Verde pointed out a vast HIV-1 pol diversity, with several subtypes and recombinant forms, being 5.2% classified as AU-pol. Thus, the aim of the present study was to improve the characterization of these AU sequences. The genomic DNA of seven HIV-1 AU pol-infected individuals were submitted to four overlapping nested-PCR fragments aiming to compose the full-length HIV-1 genome. The final classification was based on phylogenetic trees that were generated using the maximum likelihood and bootscan analysis. The genetic distances were calculated using Mega 7.0 software. Complete genome amplification was possible for two samples, and partial genomes were obtained for the other five. These two samples grouped together with a high support value, in a separate branch from the other sub-subtypes A and CRF26_A5U. No recombination was verified at bootscan, leading to the classification of a new sub-subtype A. The intragroup genetic distance from the new sub-subtype A at a complete genome was 5.2%, and the intergroup genetic varied from 8.1% to 19.0% in the analyzed fragments. Our stu...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research