Antioxidant and reducing lipid accumulation effects of rutin in Caenorhabditis elegans

In this study, the effect of rutin on fatty acid metabolism and antioxidant activity were evaluated. We found that the antioxidant capacity of rutin-treatedCaenorhabditis elegans was enhanced but the triglyceride content was significantly reduced. The reduction of fat accumulation by rutin was also confirmed by Oil Red O staining. RNA-seq analysis indicated that rutin significantly regulated the expression of seven genes related to lipid metabolism inC. elegans. Among the seven genes,acox-1.3,stdh-3, andfat-7 were associated with fatty acid metabolism. Rutin significantly reduced fat accumulation in bothfat-6 andfat-7 mutant strains but did not affect the fat storage offat-6/fat-7 double mutant, which indicated that the impact of rutin on fat storage depended onfat-6 andfat-7. These findings indicated that rutin reduced fat storage depending on the regulation of lipid metabolism-related genes expression and thereby regulating the biosynthesis of the corresponding unsaturated fatty acid.
Source: BioFactors - Category: Biochemistry Authors: Tags: Research Communication Source Type: research