Deeper below the surface —transcriptional changes in selected genes of Clostridium beijerinckii in response to butanol shock

Detailed analyses of transcriptome data obtained after butanol shock and their comparison with data from standard acetone –butanol–ethanol (ABE) fermentation revealed new findings and confirmed the expected population response. Although butanol shock resulted in upregulation of heat shock protein genes, their regulation is probably different than was assumed based on transcriptome data from standard ABE fermentatio n. While glucose uptake, glycolysis, and acidogenesis genes were downregulated after butanol shock, solventogenesis genes were upregulated. AbstractThe main bottleneck in the return of industrial butanol production from renewable feedstock through acetone –butanol–ethanol (ABE) fermentation by clostridia, such asClostridium beijerinckii, is the low final butanol concentration. The problem is caused by the high toxicity of butanol to the production cells, and therefore, understanding the mechanisms by which clostridia react to butanol shock is of key importance. Detailed analyses of transcriptome data that were obtained after butanol shock and their comparison with data from standard ABE fermentation have resulted in new findings, while confirmed expected population responses. Although butanol shock resulted in upregulation of heat shock protein genes, their regulation is different than was assumed based on standard ABE fermentation transcriptome data. While glucose uptake, glycolysis, and acidogenesis genes were downregulated after butanol shock, solventoge...
Source: MicrobiologyOpen - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research