Essential oils from < em > Artemisia < /em > species inhibit biofilm formation and the virulence of < em > Escherichia coli < /em > EPEC 2348/69

Biofouling. 2021 Feb 15:1-11. doi: 10.1080/08927014.2021.1886278. Online ahead of print.ABSTRACTEnteropathogenic Escherichia coli E2346/69 (EPEC) has caused foodborne outbreaks worldwide and the bacterium forms antimicrobial-tolerant biofilms. The anti-biofilm formation of various components of essential oils extracted from selected medicinal plants were investigated and tested on EPEC and wild strains of E. coli. Oils extracted from the family Asteraceae and their major common constituents at 0.031 and 0.062% (V/v) were found to significantly inhibit biofilm formation without affecting the growth of planktonic cells. In addition, three plants belonging to this family (Artemisia herba alba, Artemisia campestris and Artemisia absinthium) played important roles in the antimicrobial activity. Interestingly, their essential oils reduced the ability of E. coli (the EPEC and K12 strains) to form a biofilm. The crystal violet reduction assay showed that the plant extracts tested reduced biofilm formation with the inhibition of bacterial attachment up to 45% for EPEC and 70% for E. coli K-12 after 24 h treatment at 0.62 mg ml-1, demonstrating that Artemisia oils had a high anti-biofilm activity on the bacteria tested. The results indicate that the locus of enterocyte effacement (LEE) acquired by horizontal transfer promotes the formation of the attaching and effacing (A/E) lesion and increases the capacity of the photogen strain (EPEC) to form a biofilm. The chemical composition of t...
Source: Biofouling - Category: Molecular Biology Authors: Source Type: research