Monitoring of endogenous nitric oxide exhaled by pig lungs during ex-vivo lung perfusion

In the context of organ shortage for transplantation, new criteria for better organ evaluation
should be investigated. Ex-vivo lung perfusion (EVLP) allows extra-corporal lung re-conditioning and
evaluation, under controlled parameters of the organ reperfusion and mechanical ventilation. This
work reports on the interest of exhaled gas analysis during the EVLP procedure. After a 1 h cold
ischemia, the endogenous gas production by an isolated lung of nitric oxide and carbon monoxide is
simultaneously monitored in real time. The exhaled gas is analysed with two very sensitive and
selective laser spectrometers developed upon the technique of optical-feedback cavity-enhanced
absorption spectroscopy. Exhaled gas concentration measured for an ex-vivo lung is compared to the
corresponding production by the whole living pig, measured before euthanasia. On-line measurements
of the fraction of nitric oxide in exhaled gas (F E NO) in isolated lungs are reported here for the
first ...

Source: Journal of Breath Research - February 12, 2021 Category: Respiratory Medicine Authors: Thibaut Chollier, Lucile Richard, Daniele Romanini, Ang élique Brouta, Jean-Luc Martin, Cécile Moro, Raphaël Briot and Irène Ventrillard Source Type: research