Genome-wide CRISPR screens reveal a specific ligand for the glycan-binding immune checkpoint receptor Siglec-7 [Biochemistry]

Glyco-immune checkpoint receptors, molecules that inhibit immune cell activity following binding to glycosylated cell-surface antigens, are emerging as attractive targets for cancer immunotherapy. Defining biologically relevant ligands that bind and activate such receptors, however, has historically been a significant challenge. Here, we present a CRISPRi genomic screening strategy that allowed...

Source: Proceedings of the National Academy of Sciences - January 25, 2021 Category: Science Authors: Simon Wisnovsky, Leonhard Mockl, Stacy A. Malaker, Kayvon Pedram, Gaelen T. Hess, Nicholas M. Riley, Melissa A. Gray, Benȷamin A. H. Smith, Michael C. Bassik, W. E. Moerner, Carolyn R. Bertozzi Tags: Biochemistry Biological Sciences Source Type: research