Galectin ‐1–RNA interaction map reveals potential regulatory roles in angiogenesis

This study applied the iRIP‐seq methodology to study the potential role of galectin‐1 as an RNA‐binding protein. We found that galectin‐1 interacts with a large number of mRNAs, with a preference for binding near stop codons and a preference for UGCA/UGGA and GAGCAG as binding motifs. Galectin‐1 binds to the mRNAs of angiogenesis‐associated genes includingVEGFA,EGR1 andLAMA5, suggesting that galectin ‐1 may regulate angiogenesis via its mRNA‐binding activity. We further show that shLGALS1 inhibits capillary tube formation in anin vitro angiogenesis assay and alters the expression levels of several galectin ‐1‐bound angiogenesis‐associated mRNAs. These results uncover a previously unrecognized mRNA‐binding activity of galectin‐1.
Source: FEBS Letters - Category: Biochemistry Authors: Tags: RESEARCH ARTICLE Source Type: research