SPARC Related Modular Calcium Binding Protein 1 and IL-4/IL-13 Cytokines Interfere with Ca2+ Mobilization in Primary Human Keratinocytes

This study investigated the role of IL-4/IL-13 in keratinocyte responses to changes in extracellular Ca2+ and analyzed differentiation signals elicited via Ca2+ sensor, SPARC related modular calcium binding protein 1 (SMOC1). Real time dynamics of transmembrane Ca2+ influx were assessed in live keratinocytes by flow cytometry and microscopy. Exposure of keratinocytes to a high Ca2+ environment (1.3mM) triggered a rapid intracellular Ca2+ influx, while IL-4/IL-13-treated cells exhibited a significant decrease in the peak amplitude of Ca2+ influx (p
Source: Journal of Investigative Dermatology - Category: Dermatology Authors: Tags: Original Article Source Type: research