Cancers, Vol. 13, Pages 344: Integrin 31 Represses Reelin Expression in Breast Cancer Cells to Promote Invasion

In this study we demonstrate that 31 represses RELN expression to enhance breast cancer cell invasion. RELN mRNA was significantly increased upon RNAi-mediated 3 knockdown in two triple-negative breast cancer cell lines, MDA-MB-231 and SUM159. Modulation of baseline Reelin levels altered invasive potential, where enhanced Reelin expression in MDA-MB-231 cells reduced invasion, while RNAi-mediated suppression of Reelin in SUM159 cells increased invasion. Moreover, treatment of 31-expressing MDA-MB-231 cells with culture medium that was conditioned by 3 knockdown MDA-MB-231 cells led to decreased invasion. RNAi-mediated suppression of Reelin in 3 knockdown MDA-MB-231 cells mitigated this effect of conditioned-medium, identifying secreted Reelin as an inhibitor of cell invasion. These results demonstrate a novel role for 31 in repressing Reelin in breast cancer cells to promote invasion, supporting this integrin as a potential therapeutic target.
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research