Isolation and characterization of fast ‐growing green snow bacteria from coastal East Antarctica

In this study, 45 fast ‐growing bacteria were isolated from green snow in East Antarctica. Bacterial isolates, identified by 16S rRNA sequencing, showed a wide range of growth temperature from 4°C to 25°C and a high‐level of enzymatic activity. Phenotyping using Fourier‐transform infrared spectroscopy revealed a p ossible accumulation of intracellular polymers and lipids in some of the isolates. AbstractSnow microorganisms play a significant role in climate change and affecting the snow melting rate in the Arctic and Antarctic regions. While research on algae inhabiting green and red snow has been performed extensively, bacteria dwelling in this biotope have been studied to a much lesser extent. In this study, we performed 16S rRNA gene amplicon sequencing of two green snow samples collected from the coastal area of the eastern part of Antarctica and conducted genotypic and phenotypic profiling of 45 fast ‐growing bacteria isolated from these samples. 16S rRNA gene amplicon sequencing of two green snow samples showed that bacteria inhabiting these samples are mostly represented by familiesBurkholderiaceae (46.31%),Flavobacteriaceae (22.98%), andPseudomonadaceae (17.66%). Identification of 45 fast ‐growing bacteria isolated from green snow was performed using 16S rRNA gene sequencing. We demonstrated that they belong to the phylaActinobacteria andProteobacteria, and are represented by the generaArthrobacter,Cryobacterium,Leifsonia,Salinibacterium,Paeniglutamicibact...

Source: MicrobiologyOpen - December 29, 2020 Category: Microbiology Authors: Margarita Smirnova, Uladzislau Miamin, Achim Kohler, Leonid Valentovich, Artur Akhremchuk, Anastasiya Sidarenka, Andrey Dolgikh, Volha Shapaval Tags: ORIGINAL ARTICLE Source Type: research