Microencapsulation of Omeprazole by Lactobacillus acidophilus ATCC 4356 surface layer protein ...

The present study introduces a novel method for encapsulation of the acid-labile drugcalled Omeprazole using Lactobacillus acidophilus ATCC 4356 S-layer protein. Before preparing the Omeprazole suspension, a series of preliminary studies were performed on the Omeprazole powder. For this purpose, some parameters such asmelting point, IR spectrum, UV spectrum and the particle size of Omeprazole powder were investigated. The size reduction process was donein order to achieve an ideal formulation. Ultimately, the resulting powder had an average particle size of 35.516μm and it was almost uniform. After calculating the amount of S-layer protein required for complete coveringof drug particles, the effect of different factors on the drug coating processwas investigated with one at a time method. Then stability of coated Omeprazole was evaluated in acetate buffer (pH5). Finally, the maximum coat of drug particles was determined using S- layer protein of L. acidophilus ATCC 4356 at 25°C for 2h, shaking rate of 100 rpm and ratio of 2:1 forS-layer protein amount /Omeprazole Surface in Tris hydrochloride buffer medium(50 mM, pH 8). The coating of Omeprazole by the S-layer protein decreased the drug decomposition rate up to 2.223.
Source: Iranian Journal of Pharmaceutical Research - Category: Drugs & Pharmacology Source Type: research