Application of supercritical fluid chromatography for separation and quantitation of fifteen co-formulated binary anti-hypertensive medications using a single elution protocol.

Application of supercritical fluid chromatography for separation and quantitation of fifteen co-formulated binary anti-hypertensive medications using a single elution protocol. Biomed Chromatogr. 2020 Nov 23;:e5035 Authors: Pandya PA, Shah PA, Shrivastav PS Abstract A facile supercritical fluid chromatography method is proposed to analyze fifteen co-formulated binary anti-hypertensive drug combinations using a customized elution procedure. The effect of mobile phase composition, column back pressure and temperature were suitably optimized for adequate retention, analyte response and resolution. The chromatographic separation of different drug combinations was performed on DCPak P4VP (250 × 4.6 mm, 5 μm) column at 125 bar pressure and 40 °C using photo diode array detector. A linear gradient of CO2 and 0.1% formic acid in methanol provided the best elution conditions for all drug combinations. Baseline separation of the drugs was possible with resolution factor Rs ranging from 1.42-12.58. The method was validated for specificity, sensitivity, accuracy and precision, recovery and robustness. The limit of detection and limit of quantitation for aliskiren, amlodipine, atenolol, candesartan, hydrochlorothiazide, lisinopril, losartan, metoprolol, olmesartan, telmisartan and valsartan were in the range of 0.26-2.56 μg/mL and 0.77-7.75 μg/mL, respectively. The thermodynamic study revealed that interactions of the drugs with the stationa...
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Tags: Biomed Chromatogr Source Type: research