Nbnrp1 Mediates Verticillium dahliae Effector PevD1-Triggered Defense Responses by Regulating Sesquiterpenoid Phytoalexins Biosynthesis Pathway in Nicotiana benthamiana.

In this study, we further investigated PevD1-induced immune responses in both wild-type (WT) and Nbnrp1-RNAi lines through RNA-seq, in order to reveal the underlying mechanism of Nbnrp1-modulated PevD1-induced disease resistance in N. benthamiana. Results showed that Nbnrp1-RNAi lines exhibited reduced PevD1-induced immune responses, like inhibiting H2O2 accumulation and MAPK phosphorylation. To silence Nbnrp1 inhibited the expression of PevD1-induced differential expression genes (DEGs) involved in pathways associated with sesquiterpenoid and triterpenoid biosynthesis, flavone and flavonol biosynthesis, plant-pathogen interaction and phenylpropanoid biosynthesis, etc. It is worth noting that sesquiterpene phytoalexin capsidiol accumulation were obviously decreased in Nbnrp1-RNAi plants after PevD1 treatment, accompanied with the down-expression of EAS and EAH, which were two key genes related to capsidiol biosynthesis. These results suggested that Nbnrp1 mediates PevD1-induced defense responses by regulating sesquiterpenoid phytoalexins biosynthesis pathway.
PMID: 33186613 [PubMed - as supplied by publisher]

Source: Gene - November 10, 2020 Category: Genetics & Stem Cells Authors: Liang Y, Li Z, Zhang Y, Meng F, Qiu D, Zeng H, Li G, Yang X Tags: Gene Source Type: research