Sensors, Vol. 20, Pages 5525: Raman Microscopy: Progress in Research on Cancer Cell Sensing

Sensors, Vol. 20, Pages 5525: Raman Microscopy: Progress in Research on Cancer Cell Sensing Sensors doi: 10.3390/s20195525 Authors: Satheeshkumar Elumalai Stefano Managó Anna Chiara De Luca In the last decade, Raman Spectroscopy (RS) was demonstrated to be a label-free, non-invasive and non-destructive optical spectroscopy allowing the improvement in diagnostic accuracy in cancer and analytical assessment for cell sensing. This review discusses how Raman spectra can lead to a deeper molecular understanding of the biochemical changes in cancer cells in comparison to non-cancer cells, analyzing two key examples, leukemia and breast cancer. The reported Raman results provide information on cancer progression and allow the identification, classification, and follow-up after chemotherapy treatments of the cancer cells from the liquid biopsy. The key obstacles for RS applications in cancer cell diagnosis, including quality, objectivity, number of cells and velocity of the analysis, are considered. The use of multivariant analysis, such as principal component analysis (PCA) and linear discriminate analysis (LDA), for an automatic and objective assessment without any specialized knowledge of spectroscopy is presented. Raman imaging for cancer cell mapping is shown and its advantages for routine clinical pathology practice and live cell imaging, compared to single-point spectral analysis, are debated. Additionally, the combination of RS with microfluidic devices and high-...
Source: Sensors - Category: Biotechnology Authors: Tags: Review Source Type: research