Antileukemic Effect of Palladium Nanoparticles Mediated by White Tea (Camellia sinensis) Extract In Vitro and in WEHI-3B-Induced Leukemia In Vivo.

This study investigated the in vivo antileukemic activity of palladium nanoparticles ([e-mail]-NPs) mediated by white tea extract in a murine model. The cell viability effect of [e-mail]-NPs, "blank" Pd nanoparticles, and white tea extract alone was determined in murine leukemia WEHI-3B cells and normal mouse fibroblasts (3T3 cells). Apoptotic and cell cycle arrest effects of [e-mail]-NPs in WEHI-3B cells were evaluated. The effects of [e-mail]-NPs administered orally to leukemic mice at 50 and 100 mg/kg daily over 28 days were evaluated. [e-mail]-NPs reduced the viability of WHEI-3B cells with IC50 7.55 μg/ml at 72 h. Blank Pd nanoparticles and white tea extract alone had smaller effects on WHEI-3B viability and on normal fibroblasts. [e-mail]-NPs increased the proportion of Annexin V-positive WHEI-3B cells and induced G2/M cell cycle arrest. Leukemic cells in the spleen were reduced by [e-mail]-NPs with an increase in Bax/Bcl-2 and cytochrome-C protein and mRNA levels indicating the activation of the mitochondrial apoptotic pathway. These effects replicated the effects of ATRA and were not observed using blank Pd nanoparticles. [e-mail]-NPs afford therapeutic efficacy against leukemia likely to pivot on activation of the mitochondrial pathway of apoptotic signaling and hence appear attractive potential candidates for development as a novel anticancer agent.
PMID: 32922508 [PubMed]

Source: Evidence-based Complementary and Alternative Medicine - September 15, 2020 Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research