Evaluation of the analytical performance of six rapid diagnostic tests for the detection of viral hepatitis B and C in Lubumbashi, Democratic Republic of Congo.

This study aimed at assessing the effectiveness of RDTs for the detection of HBsAg and anti-HCV antibodies in order to identify infected blood donors in Lubumbashi. A total of 300 serum samples (100 HBsAg positive samples; 100 anti-HCV positive samples and 100 HBsAg and anti-HCV negative samples) were tested simultaneously using the 6 locally used RDTs and as gold standard the chemiluminescent assays for HBsAg and the RT-TMA for HCV.detection. The six evaluated RDTs demonstrated a sensitivity and a negative predictive value (NPV) of 100% whereas the specificity and positive predictive value (PPV) varied from 46% to 98.1%. SB BioLine HBsAg test performed best in this study with 100% of sensitivity, 97.1% of specificity,100% of NPV and 96.9% of PPV. Furthermore, sensitivity, specificity, NPV and PPV for SB BioLine HCV test were as follows: 100%, 98,1%, 100% and 93.9%. Therefore, SD BioLine tests (HBsAg, HCV) would be selected as the first line RDTs for the detection and the diagnostic of hepatitis B and C. They can prevent blood-borne transmission of HBV and HCV in areas with limited incomes as Lubumbashi. PMID: 32860797 [PubMed - as supplied by publisher]
Source: Journal of Virological Methods - Category: Virology Authors: Tags: J Virol Methods Source Type: research