Cell morphology impact on the set-up of electroporation protocols for in-suspension and adhered breast cancer cells.

Cell morphology impact on the set-up of electroporation protocols for in-suspension and adhered breast cancer cells. Electromagn Biol Med. 2020 Aug 07;:1-17 Authors: Vera-Tizatl CE, Talamás-Rohana P, Vera-Hernández A, Leija-Salas L, Rodríguez-Cuevas SA, Chávez-Munguía B, Vera-Tizatl AL Abstract In order to establish cancer-type-specific electroporation protocols for breast cancer, electroporation was performed in vitro in two modalities: in-suspension and adhered cells. Electroporation of cell suspensions was carried out through commercial electroporation cuvettes whereas a novel electrode for electroporation of adhered cells was designed and manufactured aimed to preserve cell structure, to provide a closer model to an in vivo scenario, and as a means to visualize the mechanical effects of electroporation on the cell membrane by using scanning electron microscopy. Electroporation protocols and electric field thresholds were predicted in silico and experimentally tuned through propidium iodide uptake and cell viability. Three breast-cancer cell lines (BT-20, MCF-7 and HCC1419) and a non-cancerous cell line (BEAS-2B) were used. Cancerous cells responded differently to electroporation depending on the electric parameters, cell histology, the cell culture modality, and the cell morphology (membrane thickness mainly), which was evaluated trough confocal and transmission electron microscopy. Particularly, it was found that electroche...
Source: Electromagnetic Biology and Medicine - Category: Physics Tags: Electromagn Biol Med Source Type: research