GSE131451 Transcriptomics revealed the impact of the translational inhibitor tigecycline on Acinetobacter baumannii

Contributors : Liping Li ; Karl A Hassan ; Ian T Paulsen ; Sasha G TetuSeries Type : Expression profiling by high throughput sequencingOrganism : Acinetobacter baumanniiWe applied RNA sequencing (RNA-Seq) to define the global changes in A. baumannii gene transcription in response to tigecycline exposure. Tigecycline treatment resulted in increased expression of genes involved in translational initiation, but decreased expression of genes involved in translational elongation, consistent with ribosomal stalling occurring. We also observed a decrease in expression of genes from the TCA cycle, respiration, cell division, and peptidoglycan synthesis, which is consistent with its action as a bacteriostatic antibiotic. Tigecycline exposure also had an unexpected effect on other resistance genes: two β-lactamases-encoding genes showed significantly reduced transcription, and interestingly, a checkerboard assay showed marginal synergy between tigecycline and β-lactam antibiotics in inhibiting the growth of A. baumannii. Tigecycline also induced increased expression of genes involved in horizont al gene transfer (HGT) and spontaneous mutation mediated by a DNA mismatch repair enzyme MutS, suggesting tigecycline may lead to increased rates of MDR development during clinical treatments.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Acinetobacter baumannii Source Type: research