Protective Role of DJ-1 in Endotoxin-induced Acute Kidney Injury.

In this study we examined the effect of DJ-1 deficiency in a murine model of endotoxin-induced AKI. Endotoxemia induced greater kidney injury in DJ-1-deficient mice. Further, DJ-1 deficiency increased renal oxidative stress associated with increased renal tubular apoptosis and with expression of death domain-associated protein (DAXX). Similar to the in vivo model, in vitro studies, using a medullary collecting duct cell line (IMCD3) and cytotoxic serum, we show that serum obtained from WT mice results in increased expression of s100A8/s100A9, DAXX, and apoptosis in DJ-1 deficient iMCD3 cells. Our findings demonstrate a novel renal protective role for renal tubular DJ-1 during endotoxemia through control of oxidative stress, renal inflammation, and DAXX-dependent apoptosis. PMID: 32715759 [PubMed - as supplied by publisher]
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research