A lateral flow immunoassay based on colored latex particles for detection of canine visceral leishmaniasis.

A lateral flow immunoassay based on colored latex particles for detection of canine visceral leishmaniasis. Acta Trop. 2020 Jul 18;:105643 Authors: Garcia VS, Guerrero SA, Gugliotta L, Gonzalez VDG Abstract Canine visceral leishmaniasis (CVL) is the major source of human visceral leishmaniasis. To control the spread of this disease, early and accurate detection of infected dogs is critical but challenging. The serological diagnosis of CVL remains problematic because there are no reliable commercially available tests. Most laboratories use enzyme-linked immunosorbent assay or the indirect immunofluorescent antibody test. These tests use Leishmania chagasi recombinant antigens K39 or K26 assembled with either gold-labelled Staphylococcus aureus protein A or protein G from Streptococcus pyogenes. In this work, we propose the development, optimization and standardization of a lateral flow immunoassay (LFIA) based on functionalized colored particles and a specific recombinant antigen, as a visual in situ method for the diagnosis of CVL. The following analysis variables were considered: i) the concentration of the latex-protein complex; ii) the dilution of the serum; iii) the composition of the employed buffers; iv) the nominal capillary flow time through the nitrocellulose membrane; v) the concentration of reagents fixed in the test and control lines; vi) the particle size of the colored latex; and vii) the conjugation method. Then, the o...
Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research