Solution structure of the zinc finger domain of human RNF144A ubiquitin ligase

In this study, the solution structure of RNF144A RING was determined using n uclear magnetic resonance. Moreover, using a metallochromic indicator, we spectrophotometrically determined the stoichiometry of zinc ions and elucidated that RNF144A RING binds two zinc atoms. This structural analysis provided the position and range of the active site of RNF144A RING at the atomic level, which contributes to the creation of artificial RING fingers having the specific ubiquitin‐conjugating enzyme (E2)‐binding capability.

Source: Protein Science - July 12, 2020 Category: Biochemistry Authors: Kazuhide Miyamoto, Kaori Migita, Kazuki Saito Tags: Protein Structure Reports Source Type: research