Molecules, Vol. 25, Pages 3135: New Dihydroxytyrosyl Esters from Dicarboxylic Acids: Synthesis and Evaluation of the Antioxidant Activity In Vitro (ABTS) and in Cell-Cultures (DCF Assay)
Molecules, Vol. 25, Pages 3135: New Dihydroxytyrosyl Esters from Dicarboxylic Acids: Synthesis and Evaluation of the Antioxidant Activity In Vitro (ABTS) and in Cell-Cultures (DCF Assay)
Molecules doi: 10.3390/molecules25143135
Authors:
Elia Roma
Elena Mattoni
Paolo Lupattelli
Seyed Sepehr Moeini
Tecla Gasperi
Roberta Bernini
Sandra Incerpi
Daniela Tofani
New dihydroxytyrosyl esters 2a, 2c–2j of dicarboxylic acids were synthesized from methyl orthoformate protected hydroxytyrosol 3 and diacyl chlorides. New compounds were characterized (HRMS, FT-IR, 1H- and 13C-NMR), and tested for antioxidant activity both in vitro (ABTS) and on L6 myoblasts and THP1 leukemic monocytes cell culture by DCF assay. According to the ABTS assay, compounds 2a, 2c–2j showed a TEAC value of antioxidant capacity up to twice that of Trolox. Very high or complete ROS protections were obtained in the cell environment where lipophilicity and rigidity of dicarboxylic structure seem to facilitate the antioxidant effect. MTT assay and proliferation test were used for assessment of cell viability. These compounds can be envisaged as a new class of preservatives for food or cosmetic products.
Source: Molecules - Category: Chemistry Authors: Elia Roma Elena Mattoni Paolo Lupattelli Seyed Sepehr Moeini Tecla Gasperi Roberta Bernini Sandra Incerpi Daniela Tofani Tags: Article Source Type: research