Molecules, Vol. 25, Pages 3111: Glycerosome of Melissa officinalis L. Essential Oil for Effective Anti-HSV Type 1

Molecules, Vol. 25, Pages 3111: Glycerosome of Melissa officinalis L. Essential Oil for Effective Anti-HSV Type 1 Molecules doi: 10.3390/molecules25143111 Authors: Vanti Ntallis Panagiotidis Dourdouni Patsoura Bergonzi Lazari Bilia Essential oils are complex mixtures of strongly active compounds, very volatile and sensitive to light, oxygen, moisture and temperature. Loading inside nanocarriers can be a strategy to increase their stability and successfully use them in therapy. In the present study, a commercial Melissa officinalis L. (Lamiaceae) essential oil (MEO) was analyzed by gas chromatography-mass spectrometry, loaded inside glycerosomes (MEO-GS) and evaluated for its anti-herpetic activity against HSV type 1. MEO-GS analyses were prepared by the thin layer evaporation method and they were characterized by light scattering techniques, determining average diameter, polydispersity index and ζ-potential. By transmission electron microscopy, MEO-GS appeared as small nano-sized vesicles with a spherical shape. MEO encapsulation efficiency inside glycerosomes, in terms of citral and β-caryophyllene, was found to be ca. 63% and 76% respectively, and MEO release from glycerosomes, performed by dialysis bag method, resulted in less than 10% within 24h. In addition, MEO-GS had high chemical and physical stability during 4 months of storage. Finally, MEO-GS were very active in inhibiting HSV type 1 infection of mammalian cel...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research