Sodium Alginate as a Novel Cryoprotective Agent for Cryopreservation of Endothelial Cells in a Closed Polytetrafluoroethylene Loop.

In this study, sodium alginate (SA) was employed as one of the main CPAs in a closed polytetrafluoroethylene (PTFE) loop used for cryopreservation with fast freezing of HUVECs. The ice crystal growth process was observed and the thermal enthalpy changes and osmolality of different solutions were tested. Moreover, the effects on cell viability and recovery were examined. The results showed that the addition of SA delayed the growth of ice crystals and decreased the number of ice crystals. Specifically, when 0.5% (w/v) SA was added to the CPAs, the cell survival increased by 10%. It is proved in this study that SA can be used as a novel CPA in combination with PTFE for the fast freezing of HUVECs, which is expected to improve the survival rate of cells and promote the exploration of protectants and cryopreservation in the future. PMID: 32552032 [PubMed - as supplied by publisher]
Source: Biopreservation and Biobanking - Category: Biomedical Science Tags: Biopreserv Biobank Source Type: research