Biological characterization of dehydrated amniotic membrane allograft: Mechanisms of action and implications for wound care

This study addresses the in vitrobiologic effects of a clinically available dehydrated amniotic membrane allograft (DAMA). The broad mechanism of action results from DAMA's biologic composition that leads to stimulation of cell migration cell proliferation, and reduction of pro ‐inflammatory cytokines. Results show that DAMA possesses growth factors and cytokines such as EGF, FGF, PDGFs, VEGF, TGF‐β, IL‐8, and TIMPs 1 and 2. Furthermore, in vitro experiments demonstrate that DAMA stimulates cell proliferation, cell migration, secretion of collagen type I, and the re duction of pro‐inflammatory cytokines IL‐1β, IL‐6, and TNF‐α. This study findings are consistent with the clinical benefits previously published for DAMA and other CTPs in chronic wounds suggesting that the introduction of DAMA to non‐healing, complex wounds helps to improve the wound mi lieu by providing essential structural components, cytokines, and growth factors to create an appropriate environment for wound healing.
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - Category: Materials Science Authors: Tags: ORIGINAL RESEARCH REPORT Source Type: research