Viruses, Vol. 12, Pages 583: The Nuclear Pore Complex: A Target for NS3 Protease of Dengue and Zika Viruses

Viruses, Vol. 12, Pages 583: The Nuclear Pore Complex: A Target for NS3 Protease of Dengue and Zika Viruses Viruses doi: 10.3390/v12060583 Authors: Luis Adrián De Jesús-González Margot Cervantes-Salazar José Manuel Reyes-Ruiz Juan Fidel Osuna-Ramos Carlos Noe Farfán-Morales Selvin Noé Palacios-Rápalo José Humberto Pérez-Olais Carlos Daniel Cordero-Rivera Arianna M. Hurtado-Monzón Fernando Ruíz-Jiménez Ana Lorena Gutiérrez-Escolano Rosa María del Ángel During flavivirus infection, some viral proteins move to the nucleus and cellular components are relocated from the nucleus to the cytoplasm. Thus, the integrity of the main regulator of the nuclear-cytoplasmic transport, the nuclear pore complex (NPC), was evaluated during infection with dengue virus (DENV) and Zika virus (ZIKV). We found that while during DENV infection the integrity and distribution of at least three nucleoporins (Nup), Nup153, Nup98, and Nup62 were altered, during ZIKV infection, the integrity of TPR, Nup153, and Nup98 were modified. In this work, several lines of evidence indicate that the viral serine protease NS2B3 is involved in Nups cleavage. First, the serine protease inhibitors, TLCK and Leupeptin, prevented Nup98 and Nup62 cleavage. Second, the transfection of DENV and ZIKV NS2B3 protease was sufficient to inhibit the nuclear ring recognition detected in mock-infected cells with the Mab414 antibody. Third, the mutant but not the active (WT) protease was un...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research