Viruses, Vol. 12, Pages 583: The Nuclear Pore Complex: A Target for NS3 Protease of Dengue and Zika Viruses
Viruses, Vol. 12, Pages 583: The Nuclear Pore Complex: A Target for NS3 Protease of Dengue and Zika Viruses
Viruses doi: 10.3390/v12060583
Authors:
Luis Adrián De Jesús-González
Margot Cervantes-Salazar
José Manuel Reyes-Ruiz
Juan Fidel Osuna-Ramos
Carlos Noe Farfán-Morales
Selvin Noé Palacios-Rápalo
José Humberto Pérez-Olais
Carlos Daniel Cordero-Rivera
Arianna M. Hurtado-Monzón
Fernando Ruíz-Jiménez
Ana Lorena Gutiérrez-Escolano
Rosa María del Ángel
During flavivirus infection, some viral proteins move to the nucleus and cellular components are relocated from the nucleus to the cytoplasm. Thus, the integrity of the main regulator of the nuclear-cytoplasmic transport, the nuclear pore complex (NPC), was evaluated during infection with dengue virus (DENV) and Zika virus (ZIKV). We found that while during DENV infection the integrity and distribution of at least three nucleoporins (Nup), Nup153, Nup98, and Nup62 were altered, during ZIKV infection, the integrity of TPR, Nup153, and Nup98 were modified. In this work, several lines of evidence indicate that the viral serine protease NS2B3 is involved in Nups cleavage. First, the serine protease inhibitors, TLCK and Leupeptin, prevented Nup98 and Nup62 cleavage. Second, the transfection of DENV and ZIKV NS2B3 protease was sufficient to inhibit the nuclear ring recognition detected in mock-infected cells with the Mab414 antibody. Third, the mutant but not the active (WT) protease was un...
Source: Viruses - Category: Virology Authors: Luis Adri án De Jesús-González Margot Cervantes-Salazar Jos é Manuel Reyes-Ruiz Juan Fidel Osuna-Ramos Carlos Noe Farf án-Morales Selvin No é Palacios-Rápalo Jos é Humberto Pérez-Olais Carlos Daniel Cordero-Rivera Arianna M. Hurtado-Monz ón Fern Tags: Article Source Type: research