GSE151087 Single-cell omics reveal human blood mononuclear CD14+ and/or CD1c+ cell heterogeneity

Contributors : Pierre Bourdely ; Kristine Vaivode ; Giorgio Anselmi ; Rodrigo N Ramos ; Yoann Missolo-Kousou ; Sofia Hidalgo ; Jimena Tosselo ; Nicolas Nunez ; Wilfrid Richer ; Anne Vincent-Salomon ; Alka Saxena ; Kristie Wood ; Alvaro Lladser ; Eliane Piaggio ; Julie Helft ; Pierre GuermonprezSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensDendritic cells (DC) are antigen presenting cells controlling T cell activation. In human, the diversity, ontogeny and functional capabilities of DC subsets are not fully understood. Here, we identified circulating CD88-CD1c+CD163+ DC (termed as DC3) as an immediate precursor of inflammatory CD88-CD14+CD1c+CD163+Fc εRI+ DC. DC3 develop via a specific pathway, independent from the cDC-restricted (CDP) and monocyte-restricted (cMoP) progenitors, and are activated by GM-CSF. As classical DC, but unlike monocytes, DC3 drove the activation of naïve T cells. In vitro, DC3 displayed a distinctive ability to prime C D8+ T cells expressing a tissue-homing signature and the epithelial homing alpha-E integrin (CD103) through transforming growth factor-b (TGF-β) signaling. In vivo, DC3 infiltrated luminal breast cancer primary tumors and DC3 infiltration correlated positively with CD8+CD103+CD69+ tissue-resident m emory T cells. Altogether, these findings define DC3 as a lineage of inflammatory DC endowed with a strong potential to regulate tumor immunity.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research