Rapid and cost-effective LC-MS/MS method for determination of hydroxycitric acid in plasma: Application in the determination of pharmacokinetics in commercial Garcinia preparations.

Rapid and cost-effective LC-MS/MS method for determination of hydroxycitric acid in plasma: Application in the determination of pharmacokinetics in commercial Garcinia preparations. Biomed Chromatogr. 2020 May 19;:e4902 Authors: Bhutani P, U R, Shivakumar HN, Prabhakar KR, Paul AT Abstract Garcinia cambogia is one of the most commonly used anti-obesity dietary supplements and hydroxycitric acid (HCA) is a major constituent in the commercial preparations of Garcinia. High doses of HCA are often consumed without much awaremess of its pharmacokinetic and toxicokinetic parameters, and hence a full understanding of HCA effects is lacking. The first step in understanding of these parameters is the availability of a reliable bioanalytical method. Herein, we present the first report on a UPLC-MS/MS method for analysis of HCA in rat plasma after simplified and cost-effective protein precipitation. Chromatographic separation of the analytes in the supernatant was achieved by HILIC (Hydrophilic interaction liquid chromatography), whereupon mass parameters were optimized and a rapid 5-minutes quantitative assay was developed. The method was highly sensitive, accurate, precise, and linear in the concentration range of 10.5-5000 ng/mL (validated according to US FDA guideline). Further, the method was successfully applied to describe the pharmacokinetic profile of HCA in rat plasma after administration of pure HCA and commercial Garcinia preparatio...
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Tags: Biomed Chromatogr Source Type: research