Cytosolic mtDNA released from pneumolysin-damaged mitochondria triggers IFN- β production in epithelial cells

Canadian Journal of Microbiology, e-First Articles. Pneumolysin (Ply) is a major virulence factor of Streptococcus pneumoniae. Ply-induced interferon- β (IFN-β) expression in host macrophages has been shown to be due to the accumulation of mitochondrial deoxyribonucleic acid (mtDNA) in the cytoplasm during S. pneumoniae infection. Our findings extend this work to show human bronchial epithelial cells that reside at the interface of inflammatory injury, BEAS-2B, adapt to local cues by altering mitochondrial states and releasing excess mtDNA. The results in this research showed that purified Ply induced the expression of IFN-β in human epithelial cells, which was accompanied by mitochondrial damage both in vivo and in vitro. The observation s also were supported by the increased mtDNA concentrations in the bronchial lavage fluid of mice infected with S. pneumoniae. In summary, our study demonstrated that Ply triggered the production of IFN-β in epithelial cells, and this response was mediated by mtDNA released from Ply-damaged mitocho ndria. It displayed an impressive modulation of IFN-β response to S. pneumoniae in epithelial cells.
Source: Canadian Journal of Microbiology - Category: Microbiology Authors: Source Type: research