Comparative label-free mass spectrometric analysis of temporal changes in the skeletal muscle proteome after impact trauma in rats.

This study aimed to characterize changes in the muscle proteome during the destruction, repair and early remodeling phases after impact trauma in male Wistar rats. Muscle tissue was collected from uninjured control rats and rats that were euthanized between 6 h to 14 d after impact injury. Muscle tissue was analyzed using unbiased, data-independent acquisition LC-MS/MS. We identified 770 reviewed proteins in the muscle tissue, 296 of which were differentially abundant between the control and injury groups (P£0.05). Around 50 proteins showed large differences (³10-fold) or a distinct pattern of abundance after injury. These included proteins that have not been previously identified in injured muscle, such as ferritin light chain 1, fibrinogen gamma chain, fibrinogen beta chain, osteolectin, murinoglobulin-1, T-kininogen 2, calcium-regulated heat stable protein 1, macrophage-capping protein, retinoid-inducible serine carboxypeptidase, ADP-ribosylation factor 4, Thy-1 membrane glycoprotein and ADP-ribosylation factor-like protein 1. Some proteins increased between 6 h and 14 d, while other proteins increased in a more delayed pattern at 7 d after injury. Bioinformatic analysis revealed that various biological processes including regulation of blood coagulation, fibrinolysis, regulation of wound healing, tissue regeneration, acute inflammatory response and negative regulation of immune effector process were enriched in injured muscle tissue. This study advances the understandin...
Source: Am J Physiol Endocri... - Category: Endocrinology Authors: Tags: Am J Physiol Endocrinol Metab Source Type: research