Molecules, Vol. 25, Pages 1699: Aggregation and Molecular Properties of β-Glucosidase Isoform II in Chayote (Sechium edule)

Molecules, Vol. 25, Pages 1699: Aggregation and Molecular Properties of β-Glucosidase Isoform II in Chayote (Sechium edule) Molecules doi: 10.3390/molecules25071699 Authors: Alberto Cruz Rodríguez Fabiola Anaid Sánchez Esperanza Eduardo Pérez-Campos María Teresa Hernández-Huerta Laura Pérez-Campos Mayoral Carlos Alberto Matias-Cervantes Alexis Martínez Barras Gabriel Mayoral-Andrade Luis Ángel Santos Pineda Aymara Judith Díaz Barrita Edgar Zenteno Carlos Romero Díaz Ruth Martínez Cruz Eduardo Pérez-Campos Mayoral Edith Alhelí Bernabé Pérez Alma Dolores Pérez Santiago María del Socorro Pina-Canseco Margarito Martínez Cruz The presence of isoforms of β-glucosidase has been reported in some grasses such as sorghum, rice and maize. This work aims to extract and characterize isoform II in β-glucosidase from S. edule. A crude extract was prepared without buffer solution and adjusted to pH 4.6. Contaminating proteins were precipitated at 4 °C for 24 h. The supernatant was purified by chromatography on carboxymethyl cellulose (CMC) column, molecular exclusion on Sephacryl S-200HR, and exchange anionic on QFF column. Electrophoretic analyzes revealed a purified enzyme with aggregating molecular complex on SDS-PAGE, Native-PAGE, and AU-PAGE. Twelve peptides fragments were identified by nano liquid chromatography-tandem mass spectrometry (nano LC-ESI-MS/MS), which presented as 61% identica...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research