Molecules, Vol. 25, Pages 1699: Aggregation and Molecular Properties of β-Glucosidase Isoform II in Chayote (Sechium edule)
Molecules, Vol. 25, Pages 1699: Aggregation and Molecular Properties of β-Glucosidase Isoform II in Chayote (Sechium edule)
Molecules doi: 10.3390/molecules25071699
Authors:
Alberto Cruz Rodríguez
Fabiola Anaid Sánchez Esperanza
Eduardo Pérez-Campos
María Teresa Hernández-Huerta
Laura Pérez-Campos Mayoral
Carlos Alberto Matias-Cervantes
Alexis Martínez Barras
Gabriel Mayoral-Andrade
Luis Ángel Santos Pineda
Aymara Judith Díaz Barrita
Edgar Zenteno
Carlos Romero Díaz
Ruth Martínez Cruz
Eduardo Pérez-Campos Mayoral
Edith Alhelí Bernabé Pérez
Alma Dolores Pérez Santiago
María del Socorro Pina-Canseco
Margarito Martínez Cruz
The presence of isoforms of β-glucosidase has been reported in some grasses such as sorghum, rice and maize. This work aims to extract and characterize isoform II in β-glucosidase from S. edule. A crude extract was prepared without buffer solution and adjusted to pH 4.6. Contaminating proteins were precipitated at 4 °C for 24 h. The supernatant was purified by chromatography on carboxymethyl cellulose (CMC) column, molecular exclusion on Sephacryl S-200HR, and exchange anionic on QFF column. Electrophoretic analyzes revealed a purified enzyme with aggregating molecular complex on SDS-PAGE, Native-PAGE, and AU-PAGE. Twelve peptides fragments were identified by nano liquid chromatography-tandem mass spectrometry (nano LC-ESI-MS/MS), which presented as 61% identica...
Source: Molecules - Category: Chemistry Authors: Alberto Cruz Rodr íguez Fabiola Anaid S ánchez Esperanza Eduardo P érez-Campos Mar ía Teresa Hernández-Huerta Laura P érez-Campos Mayoral Carlos Alberto Matias-Cervantes Alexis Mart ínez Barras Gabriel Mayoral-Andrade Luis Ángel Santos Pineda Ayma Tags: Article Source Type: research