Thirty-day experimental diabetes impairs contractility and increases fatigue resistance in rat diaphragm muscle associated with increased anti-oxidative activity.

THIRTY-DAY EXPERIMENTAL DIABETES IMPAIRS CONTRACTILITY AND INCREASES FATIGUE RESISTANCE IN RAT DIAPHRAGM MUSCLE ASSOCIATED WITH INCREASED ANTI-OXIDATIVE ACTIVITY. Can J Physiol Pharmacol. 2020 Apr 03;: Authors: Peixoto JVC, Santos ASR, Corso CR, da Silva FS, Capote A, Ribeiro CD, da Graça Azevedo BJ, Acco A, Hochmuller Fogaca RT, Dias FAL Abstract Diabetes Mellitus (DM) is a metabolic disorder which can generate tissue damage through several pathways. Alteration and dysfunction of skeletal muscle are reported including respiratory muscles, which may compromise respiratory parameters in diabetic patients. We aimed to evaluate the diaphragm muscle contractility, tissue remodeling, oxidative stress and inflammatory parameters from 30 days streptozotocin-treated rats. The diaphragm contractility was assessed using isolated muscle, tissue remodeling using histology and zymography techniques, tissue oxidative stress and inflammatory parameters by enzyme activity assay. Our data revealed in the DM group an increase in maximum tetanic force [4.82 ± 0.13 vs 4.24 ± 0.18 N/cm2 (p = 0.015)] and fatigue resistance [139.16 ± 10.78 vs 62.25 ± 4.45 seconds (p <0.001)], reduction of 35.4% in muscle trophism (p < 0.001), increased 32.6% of collagen deposition (p = 0.007), reduction of 21.3% in N-acetylglucosaminidase activity (p < 0.001), increased 246.7% of catalase activity (p = 0.002) without changes in reactive oxygen species (p = 0....
Source: Canadian Journal of Physiology and Pharmacology - Category: Drugs & Pharmacology Authors: Tags: Can J Physiol Pharmacol Source Type: research