GSE147883 Single-cell RNA sequencing of healthy and dystrophic murine skeletal muscle

Contributors : Jordi Camps ; Maurilio SampaolesiSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusFibrosis and fat replacement in the skeletal muscle is a major complication that leads to a loss of mobility in chronic muscle disorders, such as muscular dystrophy. However, our current knowledge on the in vivo properties of adipogenic stem and precursor cells remains unclear, mainly due to the high cell heterogeneity in skeletal muscles. For this purpose, we used single-cell RNA-sequencing to decomplexify interstitial cell populations in healthy and dystrophic skeletal muscles. We identified a CD142 (F3) positive cell subpopulation in mice and humans that is responsible for the inhibition of adipogenesis. Furthermore, we found a completely altered composition of interstitial cells in muscular dystrophy, with a near absence of the CD142-positive cells. The novel discovery of these adipo-regulatory cells in the skeletal muscle aids our understanding regarding the aberrant fat deposition in muscular dystrophy, paving the way for treatments that potentially sustain ambulation in patients with muscular dystrophy.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

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Studies have shown that learning and behavioural difficulties are not seen in all Duchenne muscular dystrophy (DMD) patients, and when they are reported the severity varies greatly [1 –4]; this is in contrast to the condition of progressive muscle wasting which is universal. To people living with DMD, brain comorbidities can in fact have a greater impact on the family than limited mobility [5]. There is an acute need for a better understanding of the origin of the cognitive phe notype so that better advice can be provided, learning aids can to be developed or made available, and to determine where appropriate neurops...
Source: Neuromuscular Disorders - Category: Neurology Authors: Tags: Review Source Type: research
Publication date: Available online 3 January 2020Source: Plant ScienceAuthor(s): Juliana Cotabarren, Daniela Lufrano, Mónica Graciela Parisi, Walter David ObregónAbstractProtease inhibitors (PIs) are regulatory proteins found in numerous animal tissues and fluids, plants, and microorganisms that reduce and inhibit the exacerbated and uncontrolled activity of the target proteases. Specific PIs are also effective tools for inactivating proteases involved in human diseases like arthritis, pancreatitis, hepatitis, cancer, AIDS, thrombosis, emphysema, hypertension, and muscular dystrophy among others. Plant PIs&md...
Source: Plant Science - Category: Biology Source Type: research
Conclusion These qualitative data from chronic NPPV users suggest that both M-NPPV and nasal BPAP may interfere with speaking but that speech is usually better and speaking is usually easier with M-NPPV. These findings can be explained primarily by the nature of the 2 ventilator delivery systems and their interfaces. PMID: 31306604 [PubMed - in process]
Source: American Journal of Speech-Language Pathology - Category: Speech-Language Pathology Authors: Tags: Am J Speech Lang Pathol Source Type: research
AbstractThe purpose of the study is to describe experiences of swallowing with two forms of noninvasive positive-pressure ventilation (NPPV): mouthpiece NPPV (M-NPPV) and nasal bilevel positive airway pressure (BPAP) in people with muscular dystrophy. Ten men (ages 22 –42 years;M  =  29.3; SD = 7.1) with muscular dystrophy (9 with Duchenne’s; 1 with Becker’s) completed the Eating Assessment Tool (EAT-10; Ann Otol Rhinol Laryngol 117(12):919-924 [33]) and took part in semi-structured interviews. The interviews were audio recorded, transcribed, and verified. Phenomenological...
Source: Dysphagia - Category: Speech-Language Pathology Source Type: research
See something of interest? Please share our postings with colleagues in your institutions! Spotlight September is National Preparedness Month. Disasters Happen. Prepare Now. Learn How. This week: Learn Life Saving Skills. Member Highlights: Let us shine a spotlight on the amazing work you do! NNLM MAR is always interested in learning about health outreach projects and activities that are happening in Pennsylvania, New York, New Jersey and Delaware. Share your story with us to receive a Member Highlight on the MARquee! National Network of Libraries of Medicine News Funding Available: NNLM MAR has funding available for ...
Source: NN/LM Middle Atlantic Region Blog - Category: Databases & Libraries Authors: Tags: Weekly Postings Source Type: news
In January, 2018, Academic Press published my bookPrecision Medicine and the Reinvention of Human Disease. This book has an excellent " look inside " at itsGoogle book site, which includes the Table of Contents. In addition, I thought it might be helpful to see the topics listed in the Book's index. Note that page numbers followed by f indicate figures, t indicate tables, and ge indicate glossary terms.AAbandonware, 270, 310geAb initio, 34, 48ge, 108geABL (abelson leukemia) gene, 28, 58ge, 95 –97Absidia corymbifera, 218Acanthameoba, 213Acanthosis nigricans, 144geAchondroplasia, 74, 143ge, 354geAcne, 54ge, 1...
Source: Specified Life - Category: Information Technology Tags: index jules berman jules j berman precision medicine Source Type: blogs
There are two different storms brewing in the pharmaceutical world. On the one hand there is increasing opposition to the very high prices of drugs. On the other hand there is ever more pressure to accelerate access to drugs for seriously ill patients. President Trump expressed both of these very different concerns in his January 31st meeting with pharmaceutical executives, during which he called drug prices “astronomical” while also vowing to “streamline” the process of drug approval. It is vital that the cost of new drugs not overwhelm patients and the health care system. It is also important to g...
Source: Health Affairs Blog - Category: Health Management Authors: Tags: Costs and Spending Drugs and Medical Innovation Drugs and Medical Technology accelerated approval conditional approval drug pricing Duchenne Muscular Dystrophy early access parallel track Source Type: blogs
Conclusions Feline-assisted therapy can be used in a variety of settings, and it is gaining increased popularity. Cat-assisted therapy is particularly recommended for patients who are unable to interact with large animals such as horses or are afraid of dogs.
Source: Polish Annals of Medicine - Category: Journals (General) Source Type: research
When he was 5 years old, a boy ’s father noticed he had difficulty throwing a ball, and at age 7 he was diagnosed with facioscapulohumeral muscular dystrophy (FSHD). When he was a newborn, hearing impairment had been detected via routine screening. Herein, we report follow-up hearing disorder data and speech and language acquis ition data. His hearing level was equivalent to a pure tone average of 70 dB or more, and he had been using bilateral conventional hearing aids and had undergone training at a university hospital.
Source: International Journal of Pediatric Otorhinolaryngology Extra - Category: ENT & OMF Authors: Tags: Case Report Source Type: research
Upper extremity function can limit performance of daily activities in boys with Duchenne muscular dystrophy (DMD). When arm function is decreasing, compensatory movements are used to accomplish daily activities. Movement of the trunk is often seen as a compensatory mechanism in DMD. To develop effective interventions or supportive aids it is important to get more insight in the compensatory movements of the trunk in relation to arm function and their relationship with the different disease stages.
Source: Neuromuscular Disorders - Category: Neurology Authors: Source Type: research
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