Bacteriophage amplification - a comparison of selected methods.

The objective of this study was to compare five commonly applied methods of phage amplification: (i) from a single plaque, (ii) the plate wash method, (iii) the agar culture method, (iv) the two-stage culture method, and (v) in liquid culture. All methods were tested for fifteen different phages. The results described herein indicate that there is no optimal, universal method for phage amplification, and the most effective method has to be established individually for each phage. These observations confirm the enormous diversity of bacterial viruses.
PMID: 32198027 [PubMed - as supplied by publisher]

Source: Journal of Virological Methods - March 16, 2020 Category: Virology Authors: Skaradzińska A, Ochocka M, Śliwka P, Kuźmińska-Bajor M, Skaradziński G, Friese A, Roschanski N, Murugaiyan J, Roesler U Tags: J Virol Methods Source Type: research